Gametophytes per mL - painting vs spray bottle method

[levi_44]

Hello, sorry if this is in one of the guides or has been answered already but I couldn't find it. All info I could find was for spores or by weight. I am blending the gametophytes and then counting them using a hemacytometer. How many gametophytes per mL should I be aiming for when painting onto a spool and how many when using a spray bottle? Thanks!

[toby_sheppardbloch]

@levi_44 We portion gametophytes by weight (.1 gram/100ft of seedstring) which is a more efficient/consistent way of getting even biomass applied to the seedstring.
Our method is documented here and there is a protocol for weighing gametophyte biomass at the end of the less.