A 10-step protocol detailing the proper technique for scraping kelp gametophyte biomass in laboratory culture vessels. Outlines sterile technique requirements, necessary equipment, and precise scraping methods using rubber scrapers or pasteur pipettes. Includes important contamination screening steps and specific warnings about timing scraping activities based on culture health. Features guidance on agitation techniques to prevent biomass clumping after scraping. Essential reference for nursery technicians maintaining kelp gametophyte cultures to optimize growth distribution and prevent excessive wall adherence.