{"version":"1.0","provider_name":"Ocean Farming Hub","provider_url":"https:\/\/hub.greenwave.org\/lms","author_name":"Caitlin Ueda-Valente","author_url":"https:\/\/hub.greenwave.org\/lms\/author\/caitlin_uedavalente\/","title":"Protocol for Grinding Gametophyte Biomass | Ocean Farming Hub","type":"rich","width":600,"height":338,"html":"<blockquote class=\"wp-embedded-content\" data-secret=\"gkMBX0ID59\"><a href=\"https:\/\/hub.greenwave.org\/lms\/resources\/protocol-for-grinding-gametophyte-biomass\/\">Protocol for Grinding Gametophyte Biomass<\/a><\/blockquote><iframe sandbox=\"allow-scripts\" security=\"restricted\" src=\"https:\/\/hub.greenwave.org\/lms\/resources\/protocol-for-grinding-gametophyte-biomass\/embed\/#?secret=gkMBX0ID59\" width=\"600\" height=\"338\" title=\"&#8220;Protocol for Grinding Gametophyte Biomass&#8221; &#8212; Ocean Farming Hub\" data-secret=\"gkMBX0ID59\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\" class=\"wp-embedded-content\"><\/iframe><script type=\"text\/javascript\">\n\/* <![CDATA[ *\/\n\/*! This file is auto-generated *\/\n!function(d,l){\"use strict\";l.querySelector&&d.addEventListener&&\"undefined\"!=typeof URL&&(d.wp=d.wp||{},d.wp.receiveEmbedMessage||(d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!\/[^a-zA-Z0-9]\/.test(t.secret)){for(var s,r,n,a=l.querySelectorAll('iframe[data-secret=\"'+t.secret+'\"]'),o=l.querySelectorAll('blockquote[data-secret=\"'+t.secret+'\"]'),c=new RegExp(\"^https?:$\",\"i\"),i=0;i<o.length;i++)o[i].style.display=\"none\";for(i=0;i<a.length;i++)s=a[i],e.source===s.contentWindow&&(s.removeAttribute(\"style\"),\"height\"===t.message?(1e3<(r=parseInt(t.value,10))?r=1e3:~~r<200&&(r=200),s.height=r):\"link\"===t.message&&(r=new URL(s.getAttribute(\"src\")),n=new URL(t.value),c.test(n.protocol))&&n.host===r.host&&l.activeElement===s&&(d.top.location.href=t.value))}},d.addEventListener(\"message\",d.wp.receiveEmbedMessage,!1),l.addEventListener(\"DOMContentLoaded\",function(){for(var e,t,s=l.querySelectorAll(\"iframe.wp-embedded-content\"),r=0;r<s.length;r++)(t=(e=s[r]).getAttribute(\"data-secret\"))||(t=Math.random().toString(36).substring(2,12),e.src+=\"#?secret=\"+t,e.setAttribute(\"data-secret\",t)),e.contentWindow.postMessage({message:\"ready\",secret:t},\"*\")},!1)))}(window,document);\n\/* ]]> *\/\n<\/script>\n","description":"An 18-step protocol outlining precise methods for grinding kelp gametophyte biomass in laboratory settings. Details equipment options for both small-scale (microcentrifuge tubes) and larger-scale (tissue grinder) processing. Includes critical contamination screening steps, biomass transfer techniques, and proper grinding methods to maintain cell viability. Features specific warnings about contamination management, particularly regarding ectocarpus fragmentation risks. Essential reference for nursery technicians working with kelp propagation who need to process gametophyte biomass for culture expansion or experimental applications."}