Prepare Your Nutrient Solution

Over the course of the hatchery season, you’ll need to add nutrients to your tanks to support the growth of your seed. There are two common nutrient solutions available on the market. 

For multiple seasons, GreenWave has run side-by-side experiments adding F/2 and Provasoli Enriched Seawater (PES) to our tanks. We have found no significant differences in spool quality or the number of days until outplanting. Due to its ease of use and affordability, we now solely operate with F/2 in our hatchery and feel confident in recommending it to others.

Common Nutrient Solutions
  • Provesoli’s Enriched Seawater (PES)

  • Guillard’s F/2 Nutrient Media

Guillard’s F/2 Nutrient Media

If you plan to use F/2, place your order one to two months ahead of the hatchery season to be sure it will arrive on time. In the GreenWave nursery, we use a powdered form of F/2 because it is easier to mix and it has a longer shelf life than the liquid form. As a reminder if you choose to use the liquid form, you will only want to use Part A and Part B. Part C contains silica, which can support diatom growth (a common contaminant). Both solutions can be stored, unopened, in a refrigerator until the start of the season. 

For making F/2 from the powder form, add 100g of powder per liter of sterile seawater. Dose your tanks at 0.22mL per 1L of water in the tank. You can refer to the Media and Solutions Chart for easy reference.

Based on the recommendations for adding liquid F/2 to seawater, add 0.129mL of both part A and part B per liter of water in your tank. For example, in the GreenWave hatchery, our tanks are 75L which results in a nutrient addition of 9.6mL of each solution. We have chosen to round this number to 10mL of Part A and Part B for ease of operation. 

Provesoli’s Enriched Seawater (PES)

PES is time and labor-intensive to make, and depending on the volume you need, it may take several full days in the lab to produce.

You can make your PES following the instructions outlined in the Ocean Approved Manual, and added it to your culture tanks at half-strength (10mL per L), for a total of 750mL per tank. If you choose to make your own PES – we have created a calculator tool to help determine how many liters of solution you will need for your entire hatchery season, and the corresponding recipe for that volume.

1  Berges, J.; Franklin, D.; Harrison, P. 2001. Evolution of an artificial seawater medium: Improvements in enriched seawater, artificial water over the last two decades. Journal of Phycology. 37: 1138 – 1145.